Structure of pericellular matrix around agarose-embedded chondrocytes
نویسندگان
چکیده
منابع مشابه
The dynamic structure of the pericellular matrix on living cells
Although up to several microns thick, the pericellular matrix is an elusive structure due to its invisibility with phase contrast or DIC microscopy. This matrix, which is readily visualized by the exclusion of large particles such as fixed red blood cells is important in embryonic development and in maintenance of cartilage. While it is known that the pericellular matrix which surrounds chondro...
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Appendix (Supplementary Material) Appendix A. Experimental quantification of the ratedependence of indentation hysteresis of individual chondrocytes Freshly isolated cells were probed with the nanosized and micron-sized tips at indentation rates of 200nm/s, 500nm/s, 1μm/s, 3μm/s, and 5μm/s. The area enclosed by the hysteresis loop (e.g., Fig. 6) is related to the strain energy dissipated during...
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The nanomechanical properties of individual cartilage cells (chondrocytes) and their aggrecan and collagen-rich pericellular matrix (PCM) were measured via atomic force microscope nanoindentation using probe tips of two length scales (nanosized and micron-sized). The properties of cells freshly isolated from cartilage tissue (devoid of PCM) were compared to cells that were cultured for selected...
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Chondrocytes produce large pericellular coats in vitro that can be visualized by the exclusion of particles,e.g., fixed erythrocytes, and that are removed by treatment with Streptomyces hyaluronidase, which is specific for hyaluronate. In this study, we examined the kinetics of formation of these coats and the relationship of hyaluronate and proteoglycan to coat structure. Chondrocytes were iso...
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ژورنال
عنوان ژورنال: Osteoarthritis and Cartilage
سال: 2007
ISSN: 1063-4584
DOI: 10.1016/j.joca.2007.03.023